Colicin typing of Shigella sonnei.
نویسندگان
چکیده
The colicin typing method for differentiating epidemic strains of Shigella sonnei was evaluated. Forty percent of the strains were untypable, but the method divides 60% of the strains into 15 established colicin types. The method has reasonably good reproducibility, but should not be interpreted with the same degree of confidence as some other characteristics such as serotypes. For this reason, known type strains should be included as controls in each analysis to ensure uniformity of results.
منابع مشابه
Colicin typing as an epidemiological tool in the investigation of outbreaks of Shigella sonnei.
Shigella sonnei has become the most frequently reported cause of shigellosis in the United States. Since Shigella subgroup D has no other serotypes, colicin production has been used as a basis for differentiating and identifying epidemiologically related strains. The results of colicin typing 115 cultures of S. sonnei from eight outbreaks of shigellosis occurring in widely separated regions of ...
متن کاملColicin type 7 produced by majority of Shigella sonnei isolated from Thai patients with diarrhoea
Thirty one out of 153 strains of Shigella sonnei isolated from Thai patients with diarrhoea showed antibacterial activity against S. sonnei by agar well diffusion method. All of them harbor plasmids with the genetic determination of colicin type 7 (Js) gene but without colicin E and colicin U gene. The PCR product obtained from strain 35/44 was shown to be the gene for colicin type 7 lytic prot...
متن کاملActivity spectrum of colicins produced by Shigella sonnei and genetic mechanism of colicin resistance in conspecific S. sonnei strains and Escherichia coli.
Colicin-mediated killing is an example of allelopathy, which has been found among several bacteria. Screening of 42 strains of Shigella sonnei isolated from diarrheal patients revealed that 39 (93%) S. sonnei strains were positive for colicin production against Escherichia coli DH5α. In the PCR-based detection of the colicin types, 36 (92.3%) were identified as E3, 2 (5.1%) as E3 and E8, and 1 ...
متن کاملColicine types of Shigella sonnei isolated in Hong Kong.
Two-hundred and twenty-five strains of Shigella sonnei isolated from sporadic cases of bacillary dysentery were subjected to colicine typing. The result showed that types 15 and 12 were the prevailing types in Hong Kong and two new types which did not fit in with the colicine patterns of the current types were observed. A marked increase in the numbers of strains concurrently resistant to chlor...
متن کاملShigella sonnei plasmids: evidence that a large plasmid is necessary for virulence.
Virulent form I Shigella sonnei strains contain a 120-megadalton plasmid that is absent in their form II derivatives, which are always avirulent and devoid of O side chains. In the present study, 165 biochemical and antibiotic traits were assessed, but no experimentally useful phenotype could be associated with this large form I plasmid. Therefore, the form I plasmids of several S. sonnei strai...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Applied microbiology
دوره 27 2 شماره
صفحات -
تاریخ انتشار 1974